› 7cyg

X-ray diffraction
3.2Å resolution

Crystal structure of a cysteine-pair mutant (Y113C-P190C) of a bacterial bile acid transporter before disulfide bond formation

Released:
DOI: 10.2210/pdb7cyg/pdb
PDB entry 7cyg coloured by chain, front view.
PDB entry 7cyg coloured by chain, side view.
PDB entry 7cyg coloured by chain, top view.
Source organism: Yersinia frederiksenii
Primary publication:
An engineered disulfide bridge traps and validates an outward-facing conformation in a bile acid transporter.
Wang X, Lyu Y, Ji Y, Sun Z, Zhou X
Acta Crystallogr D Struct Biol 77 108-116 (2021)
PMID: 33404530

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transporter, sodium/bile acid symporter family Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 32.67 KDa
Source organism: Yersinia frederiksenii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A380PV03 (Residues: 1-307; Coverage: 100%)
Gene name: NCTC11470_02445
Sequence domains: Sodium Bile acid symporter family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 189.605Å b: 47.164Å c: 70.681Å
α: 90° β: 100.84° γ: 90°
R-values:
R R work R free
0.265 0.263 0.301
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Substrate Specificities and Inhibition Pattern of the Solute Carrier Family 10 Members NTCP, ASBT and SOAT.
Grosser et al. (2021)