› 7d68

Electron Microscopy
3Å resolution

Cryo-EM structure of the human glucagon-like peptide-2 receptor-Gs protein complex

Released:
DOI: 10.2210/pdb7d68/pdb
PDB entry 7d68 coloured by chain, front view.
PDB entry 7d68 coloured by chain, side view.
PDB entry 7d68 coloured by chain, top view.
Source organisms:
Primary publication:
A unique hormonal recognition feature of the human glucagon-like peptide-2 receptor.
Sun W, Chen LN, Zhou Q, Zhao LH, Yang D, Zhang H, Cong Z, Shen DD, Zhao F, Zhou F, Cai X, Chen Y, Zhou Y, Gadgaard S, van der Velden WJC, Zhao S, Jiang Y, Rosenkilde MM, Xu HE, Zhang Y, Wang MW
Cell Res 30 1098-1108 (2020)
PMID: 33239759
Related structures: EMD-30590

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208188 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 43.9 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63097 (Residues: 1-3, 61-184; Coverage: 36%)
  • Canonical: P04896 (Residues: 4-67, 208-394; Coverage: 64%)
Gene names: GNAI1, GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 40.23 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62871 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody-35 Chain: N
Molecule details ›
Chain: N
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: synthetic construct
Expression system: Not provided
Glucagon-like peptide 2 Chain: P
Molecule details ›
Chain: P
Length: 33 amino acids
Theoretical weight: 3.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01275 (Residues: 146-178; Coverage: 21%)
Gene name: GCG
Sequence domains: Peptide hormone
Glucagon-like peptide 2 receptor Chain: R
Molecule details ›
Chain: R
Length: 664 amino acids
Theoretical weight: 75.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O95838 (Residues: 1-490; Coverage: 89%)
Gene name: GLP2R
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-30590
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

6 review citations

GLP-1 and GLP-2 Orchestrate Intestine Integrity, Gut Microbiota, and Immune System Crosstalk.
Abdalqadir et al. (2022)
5 more

10 mentions without citation

Targeting VIP and PACAP Receptor Signaling: New Insights into Designing Drugs for the PACAP Subfamily of Receptors.
Lu et al. (2022)
9 more