› 7jfo

Electron Microscopy
2.13Å resolution

EPYC1(49-72)-bound Rubisco

Released:
DOI: 10.2210/pdb7jfo/pdb
PDB entry 7jfo coloured by chain, front view.
PDB entry 7jfo coloured by chain, side view.
PDB entry 7jfo coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
The structural basis of Rubisco phase separation in the pyrenoid.
He S, Chou HT, Matthies D, Wunder T, Meyer MT, Atkinson N, Martinez-Sanchez A, Jeffrey PD, Port SA, Patena W, He G, Chen VK, Hughson FM, McCormick AJ, Mueller-Cajar O, Engel BD, Yu Z, Jonikas MC
Nat Plants 6 1480-1490 (2020)
PMID: 33230314
Related structures: EMD-22308

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
PDBe Complex ID:
PDB-CPX-133656 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ribulose bisphosphate carboxylase large chain Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 475 amino acids
Theoretical weight: 52.61 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P00877 (Residues: 1-475; Coverage: 100%)
Gene name: rbcL
Sequence domains:
Ribulose bisphosphate carboxylase small subunit, chloroplastic 2 Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 185 amino acids
Theoretical weight: 20.67 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P08475 (Residues: 1-185; Coverage: 100%)
Gene names: RBCS-2, RBCS2
Sequence domains: Ribulose bisphosphate carboxylase, small chain
LCI5 Chains: q, r, s, t, u, v, w, x
Molecule details ›
Chains: q, r, s, t, u, v, w, x
Length: 24 amino acids
Theoretical weight: 2.83 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
UniProt:
  • Canonical: Q94ET8 (Residues: 49-72; Coverage: 8%)
Gene name: Lci5

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SMC 8 x SMC

Experiments and Validation Details

Entry percentile scores
Resolution: 2.13Å
Relevant EMDB volumes: EMD-22308
Expression system: Not provided

Quick links

Citations

4 review citations

Get closer and make hotspots: liquid-liquid phase separation in plants.
Kim et al. (2021)
3 more

4 mentions without citation

New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields.
Adler et al. (2022)
3 more