› 7jsl

X-ray diffraction
4.51Å resolution

Crystal structure of the DNA binding domain of human transcription factor ERF in the oxidized form, in complex with double-stranded DNA ACCGGAAGTG

Released:
DOI: 10.2210/pdb7jsl/pdb
PDB entry 7jsl coloured by chain, front view.
PDB entry 7jsl coloured by chain, side view.
PDB entry 7jsl coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insight into the DNA Binding Function of Transcription Factor ERF.
Hou C, McCown C, Ivanov DN, Tsodikov OV
Biochemistry (2020)
PMID: 33175491

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121405 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ETS domain-containing transcription factor ERF Chains: E, H, J, L
Molecule details ›
Chains: E, H, J, L
Length: 123 amino acids
Theoretical weight: 14.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50548 (Residues: 22-140; Coverage: 22%)
Gene name: ERF
Sequence domains: Ets-domain
DNA (5'-D(*AP*CP*CP*GP*GP*AP*AP*GP*TP*G)-3') Chains: A, B, F, I
Molecule details ›
Chains: A, B, F, I
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*AP*CP*TP*TP*CP*CP*GP*GP*T)-3') Chains: C, D, G, K
Molecule details ›
Chains: C, D, G, K
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I222
Unit cell:
a: 127.537Å b: 128.464Å c: 174.914Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 citations in other articles

The CIC-ERF co-deletion underlies fusion-independent activation of ETS family member, ETV1, to drive prostate cancer progression.
Gupta et al. (2022)

1 mention without citation

Structural Insight into the DNA Binding Function of Transcription Factor ERF.
Hou et al. (2020)