› 7jsy

Electron crystallography
1.78Å resolution

Proteinase K soaked with I3C determined by MicroED from a single milled microcrystal

Released:
DOI: 10.2210/pdb7jsy/pdb
PDB entry 7jsy coloured by chain, front view.
PDB entry 7jsy coloured by chain, side view.
PDB entry 7jsy coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking.
Martynowycz MW, Gonen T
Structure 29 88-95.e2 (2021)
PMID: 33007196
Related structures: EMD-22463

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.93 KDa
Source organism: Parengyodontium album
Expression system: Not provided
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family

Ligands and Environments

1 bound ligand:
Ligand I3C 4 x I3C
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 1.78Å
Relevant EMDB volumes: EMD-22463
Expression system: Not provided

Quick links

Citations

5 review citations

Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.
Chua et al. (2022)
4 more

1 mention without citation

Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking.
Martynowycz et al. (2021)