› 7rsa

X-ray diffraction
1.26Å resolution

STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS

Released:
DOI: 10.2210/pdb7rsa/pdb
PDB entry 7rsa coloured by chain, front view.
PDB entry 7rsa coloured by chain, side view.
PDB entry 7rsa coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of phosphate-free ribonuclease A refined at 1.26 A.
Wlodawer A, Svensson LA, Sjölin L, Gilliland GL
Biochemistry 27 2705-17 (1988)
PMID: 3401445

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand TBU 1 x TBU
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 30.18Å b: 38.4Å c: 53.32Å
α: 90° β: 105.85° γ: 90°
R-values:
R R work R free
0.15 not available not available

Quick links

Citations

11 review citations

Cancer chemotherapy--ribonucleases to the rescue.
Leland et al. (2001)
10 more

81 mentions without citation

Do all backbone polar groups in proteins form hydrogen bonds?
Fleming et al. (2005)
80 more