› 7taa

X-ray diffraction
1.98Å resolution

FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE

Released:
DOI: 10.2210/pdb7taa/pdb
PDB entry 7taa coloured by chain, front view.
PDB entry 7taa coloured by chain, side view.
PDB entry 7taa coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Structure of the Aspergillus oryzae alpha-amylase complexed with the inhibitor acarbose at 2.0 A resolution.
Brzozowski AM, Davies GJ
Biochemistry 36 10837-45 (1997)
PMID: 9283074

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-amylase A type-1/2 Chain: A
Molecule details ›
Chain: A
Length: 478 amino acids
Theoretical weight: 52.53 KDa
Source organism: Aspergillus oryzae
UniProt:
  • Canonical: P0C1B3 (Residues: 22-499; Coverage: 100%)
Gene names: AO090023000944, AO090120000196, Taa-G1, Taa-G2, amy1, amy2, amyI, amyII
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ABC 1 x ABC
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 51.04Å b: 67.183Å c: 133.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.236

Quick links

Citations

7 review citations

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
MacGregor et al. (2001)
6 more

33 mentions without citation

Structure of allergens and structure based epitope predictions.
Dall'antonia et al. (2014)
32 more