› 8cat

X-ray diffraction
2.5Å resolution

The NADPH binding site on beef liver catalase

Released:
DOI: 10.2210/pdb8cat/pdb
PDB entry 8cat coloured by chain, front view.
PDB entry 8cat coloured by chain, side view.
PDB entry 8cat coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The NADPH binding site on beef liver catalase.
Fita I, Rossmann MG
Proc Natl Acad Sci U S A 82 1604-8 (1985)
PMID: 3856839

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chains: A, B
Molecule details ›
Chains: A, B
Length: 506 amino acids
Theoretical weight: 57.66 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P00432 (Residues: 2-507; Coverage: 96%)
Gene name: CAT
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand NDP 2 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 142Å b: 142Å c: 103.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.191 not available
Expression system: Not provided

Quick links

Citations

11 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
10 more

9 mentions without citation

The widespread role of non-enzymatic reactions in cellular metabolism.
Keller et al. (2015)
8 more