› 8rnt

X-ray diffraction
1.8Å resolution

STRUCTURE OF RIBONUCLEASE T1 COMPLEXED WITH ZINC(II) AT 1.8 ANGSTROMS RESOLUTION: A ZN2+.6H2O.CARBOXYLATE CLATHRATE

Released:
DOI: 10.2210/pdb8rnt/pdb
PDB entry 8rnt coloured by chain, front view.
PDB entry 8rnt coloured by chain, side view.
PDB entry 8rnt coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Structure of ribonuclease T1 complexed with zinc(II) at 1.8 A resolution: a Zn2+.6H2O.carboxylate clathrate.
Ding J, Choe HW, Granzin J, Saenger W
Acta Crystallogr B 48 ( Pt 2) 185-91 (1992)
PMID: 1515106

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Aspergillus oryzae
Expression system: Not provided
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 48.71Å b: 46.51Å c: 41.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 not available not available
Expression system: Not provided

Quick links

Citations

1 citations in other articles

Flexibility of metal binding sites in proteins on a database scale.
Babor et al. (2005)

2 mentions without citation

Multiple structural alignment by secondary structures: algorithm and applications.
Dror et al. (2003)
1 more