Electron Microscopy Data Bank

EMD-0078

Helical reconstruction
8.0 Å

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Deposition: 26/06/2018
Map released: 12/09/2018
Last modified: 19/09/2018

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0078

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Wide Pick Filament from Pick's disease brain

EMD-0078

Helical reconstruction
8.0 Å
EMD-0078

3D View Gallery

Deposition: 26/06/2018
Map released: 12/09/2018
Last modified: 19/09/2018

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

Method: Helical reconstruction
Aggregation State: Tissue

Summary:

Specimen preparation
Microscopy
Image processing
Map

Specimen preparation [1]

Concentration: 0.5 mg/mL
Details: Dispersed filaments

Buffer

pH: 7.4
Buffer components [3]:

Name	Formula	Concentration
-	Tris-HCl	50.0 mM
-	NaCl	150.0 mM
Amphipol	A8-35	0.02 %

Vitrification

Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV

Microscopy [1]

Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.7 µm - 2.8 µm

Specialist optics

Energy filter

Name: GIF Quantum LS

Image Recording [1]

Detector model: GATAN K2 QUANTUM (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 1.06 e/Å²

Image processing [1]

Final reconstruction

Resolution: 8.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 3003

Applied Symmetry

ΔΖ: 4.7 Å
ΔΦ: -0.6°
Axial symmetry: C2

Software [1]

Name	Version	Details
RELION	2.1	-

Final angle assignment

Type: NOT APPLICABLE

CTF correction

Software [1]

Name	Version	Details
Gctf	1.06	-

Map

Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Geometry

	X	Y	Z
Dimensions	180	180	180
Origin	0	0	0
Spacing	180	180	180
Voxel size	3.45 Å	3.45 Å	3.45 Å

Contour list

Primary	Level	Source
True	0.17	AUTHOR