EMD-0109
Asymmetric region of the Membrane Attack Complex in the closed conformation
EMD-0109
Single-particle5.9 Å
Deposition: 06/07/2018
Map released: 19/12/2018
Last modified: 26/12/2018
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscope: FEI TITAN KRIOS
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal magnification: 59000.0
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal magnification: 59000.0
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Image Recording
[1]
Detector model:
FEI FALCON II (4k x 4k)
Detector mode: COUNTING
Number of grids: 8
Number of real images: 13009
Average exposure time: 2.0 s
Average electron dose per image: 50.0 e/Å2
Detector mode: COUNTING
Number of grids: 8
Number of real images: 13009
Average exposure time: 2.0 s
Average electron dose per image: 50.0 e/Å2
Final
reconstruction
Resolution: 5.9
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 53167
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 53167
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
RELION | - | - |
⦨ Initial angle
assignment
⦩ Final angle assignment
Particle selection
[1]
Selected | Ref. model | Method | Software | Details |
---|---|---|---|---|
288366 | - | - | - | - |
Final 3D classification
Software
[1]
Name | Version | Details |
---|---|---|
RELION | - | - |
CTF correction
Software
[1]
Name | Version | Details |
---|---|---|
CTFFIND | 4 | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 360 | 360 | 360 |
Origin | 0 | 0 | 0 |
Spacing | 360 | 360 | 360 |
Voxel size | 1.384 Å | 1.384 Å | 1.384 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.06 | AUTHOR |