EMD-0109

Single-particle
5.9 Å
EMD-0109 Deposition: 06/07/2018
Map released: 19/12/2018
Last modified: 26/12/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0109

Asymmetric region of the Membrane Attack Complex in the closed conformation

EMD-0109

Single-particle
5.9 Å
EMD-0109 Deposition: 06/07/2018
Map released: 19/12/2018
Last modified: 26/12/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal magnification: 59000.0
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Detector mode: COUNTING
Number of grids: 8
Number of real images: 13009
Average exposure time: 2.0 s
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 5.9 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 53167
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-3134
Details:Low pass filtered to 60 A
Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION - -
Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION - -
Particle selection [1]
Selected Ref. model Method Software Details
288366 - - - -
Final 3D classification
Software [1]
Name Version Details
RELION - -
CTF correction
Software [1]
Name Version Details
CTFFIND 4 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Geometry
X Y Z
Dimensions 360 360 360
Origin 0 0 0
Spacing 360 360 360
Voxel size 1.384 Å 1.384 Å 1.384 Å
Contour list
Primary Level Source
True 0.06 AUTHOR