EMD-1097

Subtomogram averaging
85.0 Å
EMD-1097
3D View Gallery
Deposition: 13/09/2004
Map released: 23/09/2006
Last modified: 26/05/2011
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1097
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Nuclear pore complex structure and dynamics revealed by cryoelectron tomography.

EMD-1097

Subtomogram averaging
85.0 Å
EMD-1097
3D View Gallery
Deposition: 13/09/2004
Map released: 23/09/2006
Last modified: 26/05/2011
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Subtomogram averaging
Aggregation State: Particle
Specimen preparation [1]
Buffer
Details: 50 mM Tris pH 7.6, 25 mM KCl, 5mM MgCl2, 50 mM sucrose
Vitrification
Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER
Details: Vitrification instrument: Rudolf Gatz plunger
Microscopy [1]
Microscope: FEI/PHILIPS CM300FEG/T
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.0 mm
Nominal magnification: 17500.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Temperature
Average: 90 K
Specialist optics
Energy filter
Name: GIF2000
Lower energy threshold: 0.0 eV
Upper energy threshold: 20.0 eV
Image Recording [1]
Detector category: CCD
Detector model: GATAN MULTISCAN
Tilt Series [1]
Axis 1 Axis 2
Min. Max. Inc. Min. Max. Inc. Rotation
- 63 ° - - - - -
Image processing [1]
Details: Average number of projections used in the 3D reconstructions: 267.
Final reconstruction
Resolution: 85.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details: 3D reconstruction by averaging of subtomograms
Software [1]
Name Version Details
TOM - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D structure of the Dictyostelium nuclear pore complex obtained by Cryoelectron Tomography
Details: ::::EMDATABANK.org::::EMD-1097::::
⬡ Geometry
X Y Z
Dimensions 128 128 128
Origin 0 0 0
Spacing 128 128 128
Voxel size 16.4 Å 16.4 Å 16.4 Å
Contour list
Primary Level Source
True 1.22 -