EMD-1282

Single-particle
22.0 Å
EMD-1282
3D View Gallery
Deposition: 27/10/2006
Map released: 28/10/2006
Last modified: 26/05/2011
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EMD-1282
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EMICSS annotations (xml)

Molecular architecture and conformational flexibility of human RNA polymerase II.

EMD-1282

Single-particle
22.0 Å
EMD-1282
3D View Gallery
Deposition: 27/10/2006
Map released: 28/10/2006
Last modified: 26/05/2011
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.01 mg/mL
Buffer
pH: 7.9
Details: 200mM (NH4)2SO4, 25mM Hepes, 0.2mM EDTA, 0.05% NP-40
Staining
Type: NEGATIVE
Details: Cryo-negative staining in a saturated solution of ammonium molybdate neutralized to a pH of 7.2. with 10 N NaOH
Grid
Details: 400 mesh copper grid
Vitrification
Cryogen name: ETHANE
Chamber temperature: 90 K
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.2 mm
Nominal defocus: 1.2 µm - 3.6 µm
Nominal magnification: 50000.0
Calibrated magnification: 50280.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side entry
Temperature
Average: 90 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 6.3 µm
Number of real images: 19
Average electron dose per image: 17 e/Å2
Old range: 1.0
Bits per pixel: 14.0
Image processing [1]
Final reconstruction
Resolution: 22.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 6238
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
FREALIGN - -
CTF correction
Details:Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Mixed population, before classification
Details: ::::EMDATABANK.org::::EMD-1282::::
⬡ Geometry
X Y Z
Dimensions 120 120 120
Origin -60 -60 -60
Spacing 120 120 120
Voxel size 2.5 Å 2.5 Å 2.5 Å
Contour list
Primary Level Source
True 0.0661 -