EMD-1615

Single-particle
14.4 Å
EMD-1615
3D View Gallery
Deposition: 23/04/2009
Map released: 10/09/2010
Last modified: 11/12/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1615
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Three-dimensional structure of YidC bound to the translating ribosome

EMD-1615

Single-particle
14.4 Å
EMD-1615
3D View Gallery
Deposition: 23/04/2009
Map released: 10/09/2010
Last modified: 11/12/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Grid
Details: 200 mesh copper
Vitrification
Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER
Details: Vitrification instrument: Plunger
Microscopy [1]
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 1.0 µm - 4.0 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 12.7 µm
Average electron dose per image: 15 e/Å2
Bits per pixel: 16.0
Image processing [1]
Final reconstruction
Resolution: 14.4 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 24395
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Imagic-5 Spider - -
CTF correction
Details:Each image
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: This map shows YidC bound to the tunnel exit region of an ribosome nascent chain complex
Details: ::::EMDATABANK.org::::EMD-1615::::
⬡ Geometry
X Y Z
Dimensions 108 108 108
Origin 0 0 0
Spacing 108 108 108
Voxel size 3.76 Å 3.76 Å 3.76 Å
Contour list
Primary Level Source
True 40.0 AUTHOR