EMD-1776

Single-particle
20.0 Å
EMD-1776
3D View Gallery
Deposition: 31/08/2010
Map released: 10/09/2010
Last modified: 19/09/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1776
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

The eye lens chaperone alphaB-crystallin forms defined globular, 24meric assemblies

EMD-1776

Single-particle
20.0 Å
EMD-1776
3D View Gallery
Deposition: 31/08/2010
Map released: 10/09/2010
Last modified: 19/09/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.05 mg/mL
Buffer
pH: 7.4
Details: 137mM NaCl, 2.7mM KCl, 12 mM PBS
Staining
Type: NEGATIVE
Details: Samples were negatively stained for 30s using Ammonium Molybdate solution pH 5.5
Grid
Details: 300 mesh carbon-coated copper grids
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: JEOL 100CX
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: OTHER
Acceleration voltage: 100 kV
Nominal CS: 2.8 mm
Nominal defocus: 0.9 µm - 1.2 µm
Nominal magnification: 50000.0
Specimen holder model: JEOL
Specimen holder details: Side entry
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 1.69 µm
Number of real images: 11
Bits per pixel: 16.0
Image processing [1]
Details: After correction of contrast transfer function by phase flipping the reconstructions have been performed by iterative cycles of MRA, MSA, classification and angular reconstitution of the obtained class averages. See also publication Peschek et al., 2009. The eye lens chaperone alpha-crystallin forms defined globular assemblies. PNAS 106
Final reconstruction
Resolution: 20.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 2565
Algorithm: OTHER
Details: Final maps were calculated from 40 class averages
⌯ Applied Symmetry
Point group: T
Software [1]
Name Version Details
Imagic - -
Final 2D classification
Number of classes: 40
CTF correction
Details:Phase flipping of each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: This is a map of human alphaB crystallin
Details: ::::EMDATABANK.org::::EMD-1776::::
⬡ Geometry
X Y Z
Dimensions 150 150 150
Origin 0 0 0
Spacing 150 150 150
Voxel size 1.69 Å 1.69 Å 1.69 Å
Contour list
Primary Level Source
True 0.081 AUTHOR