EMD-1883

Single-particle
20.9 Å
EMD-1883
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Deposition: 22/02/2011
Map released: 21/04/2011
Last modified: 21/04/2011
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EMD-1883
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EMICSS annotations (xml)

Three-dimensional structure of the RNA polymerase II-Iwr1 complex

EMD-1883

Single-particle
20.9 Å
EMD-1883
3D View Gallery
Deposition: 22/02/2011
Map released: 21/04/2011
Last modified: 21/04/2011
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.1 mg/mL
Buffer
pH: 7.25
Details: 5 mM HEPES, 40 mM Ammonium sulfate, 10 microM Zinc chloride, 10 mM DTT
Vitrification
Cryogen name: ETHANE
Chamber humidity: 95%
Chamber temperature: 80 K
Instrument: OTHER
Method: Blot for 10 s before plunging
Details: Vitrification instrument: FEI Vitrobot
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.5 µm - 3.5 µm
Nominal magnification: 67000.0
Specimen holder model: OTHER
Specimen holder details: Multispecimen holder
Temperature
Average: 80 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Number of real images: 35
Average electron dose per image: 25 e/Å2
Image processing [1]
Details: Particles were selected using Signature and thereafter checked manually
Final reconstruction
Resolution: 20.9 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 25206
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER - -
⦩ Final angle assignment
Details: SPIDER
CTF correction
Details:Defocus groups
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: This is a map of the RNA Polymerase II-Iwr1 complex.
Details: ::::EMDATABANK.org::::EMD-1883::::
⬡ Geometry
X Y Z
Dimensions 70 70 70
Origin 0 0 0
Spacing 70 70 70
Voxel size 3.71 Å 3.71 Å 3.71 Å
Contour list
Primary Level Source
True 0.0005 AUTHOR