EMD-1902

Single-particle
16.5 Å
EMD-1902
3D View Gallery
Deposition: 07/06/2011
Map released: 03/10/2012
Last modified: 10/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1902
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

The HSV-1 UL17 protein is the second constituent of the capsid vertex specific component (CVSC) required for DNA packaging and retention.

EMD-1902

Single-particle
16.5 Å
EMD-1902
3D View Gallery
Deposition: 07/06/2011
Map released: 03/10/2012
Last modified: 10/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: 500 mM NaCl, 10 mM Tris, 1 mM EDTA
Vitrification
Cryogen name: ETHANE
Chamber humidity: 85%
Instrument: FEI VITROBOT MARK III
Method: 7.5 second blot before plunging
Details: Vitrification instrument: Vitrobot mark III
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.63 µm - 2.73 µm
Nominal magnification: 29000.0
Calibrated magnification: 30000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 48
Bits per pixel: 8.0
Image processing [1]
Final reconstruction
Resolution: 16.5 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 3973
Algorithm: OTHER
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
AUTO3DEM - -
CTF correction
Details:Phase flip each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Surface rendered view of HSV-1 C-capsid
Details: ::::EMDATABANK.org::::EMD-1902::::
⬡ Geometry
X Y Z
Dimensions 642 642 642
Origin 0 0 0
Spacing 642 642 642
Voxel size 2.12 Å 2.12 Å 2.12 Å
Contour list
Primary Level Source
True 0.6 AUTHOR