EMD-1904
Labeling and Localization of the Herpes Simplex Virus Capsid Protein UL25 and Its Interaction with the Two Triplexes Closest to the Penton.
EMD-1904
Single-particle13.7 Å
Deposition: 07/06/2011
Map released: 03/10/2012
Last modified: 10/10/2012
Buffer
pH: 7.5
Details: 500 mM NaCl, 10 mM Tris, 1 mM EDTA
Details: 500 mM NaCl, 10 mM Tris, 1 mM EDTA
Vitrification
Cryogen name: OTHER
Instrument: FEI VITROBOT MARK III
Details: Vitrification instrument: Vitrobot mark III. Cryogen was an equal mix of ethane-propane.
Instrument: FEI VITROBOT MARK III
Details: Vitrification instrument: Vitrobot mark III. Cryogen was an equal mix of ethane-propane.
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal magnification: 29000.0
Calibrated magnification: 30000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal magnification: 29000.0
Calibrated magnification: 30000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Image Recording
[1]
Detector category:
FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Bits per pixel: 8.0
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Bits per pixel: 8.0
Final
reconstruction
Resolution: 13.7
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
I
Software
[1]
Name | Version | Details |
---|---|---|
AUTO3DEM | - | - |
CTF correction
Details:Phase flip each particle
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Surface rendered view of HSV-1 C-capsid where protein UL25 has been genetically tagged with GFP at amino acid 50.
Details: ::::EMDATABANK.org::::EMD-1904::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Surface rendered view of HSV-1 C-capsid where protein UL25 has been genetically tagged with GFP at amino acid 50.
Details: ::::EMDATABANK.org::::EMD-1904::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 642 | 642 | 642 |
Origin | 0 | 0 | 0 |
Spacing | 642 | 642 | 642 |
Voxel size | 2.12 Å | 2.12 Å | 2.12 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.1 | AUTHOR |