EMD-1905

Single-particle
14.7 Å
EMD-1905
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Deposition: 07/06/2011
Map released: 03/10/2012
Last modified: 10/10/2012
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1905
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3D volume (map.gz) Experimental metadata (xml)
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EMICSS annotations (xml)

Residues of the UL25 Protein of Herpes Simplex Virus That Are Required for Its Stable Interaction with Capsids.

EMD-1905

Single-particle
14.7 Å
EMD-1905
3D View Gallery
Deposition: 07/06/2011
Map released: 03/10/2012
Last modified: 10/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: 500 mM NaCl, 10 mM Tris, 1 mM EDTA
Vitrification
Cryogen name: ETHANE
Chamber humidity: 85%
Instrument: FEI VITROBOT MARK III
Method: 6s blot prior to plunging
Details: Vitrification instrument: Vitrobot mark III
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.25 µm - 3.73 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 83
Bits per pixel: 8.0
Image processing [1]
Final reconstruction
Resolution: 14.7 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 4865
Algorithm: OTHER
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
AUTO3DEM - -
CTF correction
Details:Phase flip each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Surface view of HSV-1 C-capsid where the UL25 protein has been genetically tagged with a tandem affinity purification (TAP) tag at its N-terminus.
Details: ::::EMDATABANK.org::::EMD-1905::::
⬡ Geometry
X Y Z
Dimensions 310 310 310
Origin 0 0 0
Spacing 310 310 310
Voxel size 2.54 Å 2.54 Å 2.54 Å
Contour list
Primary Level Source
True 0.8 AUTHOR