EMD-1951
Structure of the CS1 pilus of enterotoxigenic Escherichia coli reveals structural polymorphism
EMD-1951
Helical reconstruction20.0 Å
Deposition: 24/08/2011
Map released: 03/10/2012
Last modified: 04/05/2016
Microscope: FEI TECNAI 12
Illumination mode: OTHER
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 80 kV
Nominal CS: 2 mm
Nominal defocus: 1.0 µm - 2.0 µm
Nominal magnification: 30000.0
Calibrated magnification: 30000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Specimen holder details: Eucentric
Alignment procedure: LEGACY (Astigmatism: objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Details: Low dose mode
Illumination mode: OTHER
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 80 kV
Nominal CS: 2 mm
Nominal defocus: 1.0 µm - 2.0 µm
Nominal magnification: 30000.0
Calibrated magnification: 30000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Specimen holder details: Eucentric
Alignment procedure: LEGACY (Astigmatism: objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Details: Low dose mode
Image Recording
[1]
Detector category:
FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON COOLSCAN
Sampling interval: 4.16 µm
Number of real images: 10
Average electron dose per image: 10 e/Å2
Detector model: KODAK SO-163 FILM
Scanner: NIKON COOLSCAN
Sampling interval: 4.16 µm
Number of real images: 10
Average electron dose per image: 10 e/Å2
Details: Pilus segments were selected using the helixboxer program in SPIDER.
Final
reconstruction
Resolution: 20.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details:
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details:
⌯ Applied Symmetry
ΔΖ:
8.7
Å
ΔΦ: 113°
ΔΦ: 113°
Software
[1]
Name | Version | Details |
---|---|---|
SPIDER, IHRSR | - | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: CS1-Group 1
Details: ::::EMDATABANK.org::::EMD-1951::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: CS1-Group 1
Details: ::::EMDATABANK.org::::EMD-1951::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 100 | 100 | 100 |
Origin | -50 | -50 | -50 |
Spacing | 100 | 100 | 100 |
Voxel size | 4.16 Å | 4.16 Å | 4.16 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 4.16 | AUTHOR |