EMD-1951

Helical reconstruction
20.0 Å
EMD-1951 Deposition: 24/08/2011
Map released: 03/10/2012
Last modified: 04/05/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1951

Structure of the CS1 pilus of enterotoxigenic Escherichia coli reveals structural polymorphism

EMD-1951

Helical reconstruction
20.0 Å
EMD-1951 Deposition: 24/08/2011
Map released: 03/10/2012
Last modified: 04/05/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Concentration: 0.2 mg/mL
Buffer
pH: 7.4
Details: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, 10 mM EDTA
Staining
Type: NEGATIVE
Details: 2% uranyl acetate
Grid
Details: Glow discharged continuous carbon coated grids
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: OTHER
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 80 kV
Nominal CS: 2 mm
Nominal defocus: 1.0 µm - 2.0 µm
Nominal magnification: 30000.0
Calibrated magnification: 30000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Specimen holder details: Eucentric
Alignment procedure: LEGACY (Astigmatism: objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Details: Low dose mode
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON COOLSCAN
Sampling interval: 4.16 µm
Number of real images: 10
Average electron dose per image: 10 e/Å2
Image processing [1]
Details: Pilus segments were selected using the helixboxer program in SPIDER.
Final reconstruction
Resolution: 20.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details:
Applied Symmetry
ΔΖ: 8.7 Å
ΔΦ: 113°
Software [1]
Name Version Details
SPIDER, IHRSR - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: CS1-Group 1
Details: ::::EMDATABANK.org::::EMD-1951::::
Geometry
X Y Z
Dimensions 100 100 100
Origin -50 -50 -50
Spacing 100 100 100
Voxel size 4.16 Å 4.16 Å 4.16 Å
Contour list
Primary Level Source
True 4.16 AUTHOR