EMD-2025

Single-particle
15.0 Å
EMD-2025
3D View Gallery
Deposition: 06/01/2012
Map released: 23/01/2013
Last modified: 08/10/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2025
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

CryoEM 3D reconstruction of full Triatoma Virus

EMD-2025

Single-particle
15.0 Å
EMD-2025
3D View Gallery
Deposition: 06/01/2012
Map released: 23/01/2013
Last modified: 08/10/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Details: NMT (10 mM NaCl, 1 mM MgCl2, 50 mM Tris-HCl)
Grid
Details: 400 mesh quantifoil grid
Vitrification
Cryogen name: ETHANE
Instrument: OTHER
Details: Vitrification instrument: Vitrobot FEI
Microscopy [1]
Microscope: JEOL 2010F
Illumination mode: OTHER
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 1.4 mm
Nominal defocus: 1.2 µm - 4.0 µm
Nominal magnification: 40000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side entry liquid nitrogen-cooled cryo specimen holder
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 25
Image processing [1]
Final reconstruction
Resolution: 15.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1716
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
EM3DR2 - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Full TrV particle 3D reconstruction
Details: ::::EMDATABANK.org::::EMD-2025::::
⬡ Geometry
X Y Z
Dimensions 255 255 255
Origin -127 -127 -127
Spacing 255 255 255
Voxel size 1.75 Å 1.75 Å 1.75 Å
Contour list
Primary Level Source
True 37.0 AUTHOR