EMD-2070

Single-particle
16.0 Å
EMD-2070 Deposition: 06/04/2012
Map released: 17/04/2013
Last modified: 26/03/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2070

Octameric structure of IMPDH from Pseudomonas aeruginosa in presence of Mg-ATP

EMD-2070

Single-particle
16.0 Å
EMD-2070 Deposition: 06/04/2012
Map released: 17/04/2013
Last modified: 26/03/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 8.0
Details: 20 mM potassium phosphate pH 8 and 0.1 M KCl
Grid
Details: Quantifoil R2.2, glow discharged
Vitrification
Cryogen name: ETHANE
Chamber humidity: 98%
Chamber temperature: 102.15 K
Instrument: GATAN CRYOPLUNGE 3
Method: Blot for 1 second
Microscopy [1]
Microscope: JEOL 2200FS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.4 µm - 2.5 µm
Nominal magnification: 100000.0
Calibrated magnification: 130137.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: 626 Gatan cryo-Holder
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 200,000 times magnification, Electron beam tilt params: )
Details: Image acquisition is done directly on the CCD camera. It explains why the nominal magnification is 100000 and calibrated magnification 130137.
Temperature
Minimum: 93.15 K
Average: 95.15 K
Maximum: 96.15 K
Specialist optics
Energy filter
Name: JEOL
Lower energy threshold: 0.0 eV
Upper energy threshold: 20.0 eV
Image Recording [1]
Detector category: CCD
Detector model: GENERIC GATAN (4k x 4k)
Number of real images: 80
Average electron dose per image: 18 e/Å2
Details: Images directly recorded with CCD camera
Image processing [1]
Details: The particles were selected using a semi-automatic selection program (Boxer).
Final reconstruction
Resolution: 16.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 22577
Details:
Applied Symmetry
Point group: D4
Software [1]
Name Version Details
IMAGIC, FREALIGN - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of IMPDH from Pseudomonas Aeruginosa in presence of Mg-ATP
Details: ::::EMDATABANK.org::::EMD-2070::::
Geometry
X Y Z
Dimensions 150 150 150
Origin 0 0 0
Spacing 150 150 150
Voxel size 2.3 Å 2.3 Å 2.3 Å
Contour list
Primary Level Source
True 52.0 AUTHOR