EMD-2077

Single-particle
9.7 Å
EMD-2077
3D View Gallery
Deposition: 19/04/2012
Map released: 21/11/2012
Last modified: 09/01/2013
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2077
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in AMPPNP state.

EMD-2077

Single-particle
9.7 Å
EMD-2077
3D View Gallery
Deposition: 19/04/2012
Map released: 21/11/2012
Last modified: 09/01/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 6.8
Details: 80 mM PIPES, 5 mM MgCl2, 1 mM EGTA, 5mM AMPPNP
Grid
Details: 400 mesh holey carbon grids
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK I
Method: chamber at 24 degrees C, blot 2.5 sec
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 0.7 µm - 2.2 µm
Nominal magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 150,000 times magnification, Electron beam tilt params: )
Temperature
Average: 90 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 46
Average electron dose per image: 18 e/Å2
Bits per pixel: 8.0
Image processing [1]
Details: The particles were selected along individual microtubules.
Final reconstruction
Resolution: 9.7 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 3587
Details: Approximately 50,000 asymmetric units were averaged in the final reconstruction.
Software [1]
Name Version Details
SPIDER, FREALIGN - -
CTF correction
Details:FREALIGN
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D reconstruction of microtubule-bound human kinesin-5 motor domain with AMPPNP bound in the nucleotide-binding site
Details: ::::EMDATABANK.org::::EMD-2077::::
⬡ Geometry
X Y Z
Dimensions 45 45 45
Origin 0 0 0
Spacing 45 45 45
Voxel size 2.8 Å 2.8 Å 2.8 Å
Contour list
Primary Level Source
True 1.1 AUTHOR