EMD-2096

Single-particle
4.5 Å
EMD-2096
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Deposition: 10/05/2012
Map released: 13/03/2013
Last modified: 03/07/2013
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2096
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

Cryo-EM structure of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon at near-atomic resolution

EMD-2096

Single-particle
4.5 Å
EMD-2096
3D View Gallery
Deposition: 10/05/2012
Map released: 13/03/2013
Last modified: 03/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.7 mg/mL
Buffer
pH: 7.6
Details: 50mM Tris-HCl, 0.025mM FAD
Grid
Details: Freshly glow discharged Quantifoil holey grid (1 micrometer holes)
Vitrification
Cryogen name: ETHANE
Chamber humidity: 70%
Chamber temperature: 103 K
Instrument: FEI VITROBOT MARK I
Method: blotting 2.5 seconds before plunging
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.69 µm - 2.8 µm
Nominal magnification: 59000.0
Calibrated magnification: 61400.0
Specimen holder model: OTHER
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 120,000 times magnification, Electron beam tilt params: )
Temperature
Minimum: 77 K
Average: 79 K
Maximum: 80 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Number of real images: 101
Average electron dose per image: 15 e/Å2
Bits per pixel: 8.0
Image processing [1]
Details: EMAN2
Final reconstruction
Resolution: 4.5 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 84000
Details:
⌯ Applied Symmetry
Point group: T
Software [1]
Name Version Details
EMAN2 - -
Final 2D classification
Number of classes: 2134
CTF correction
Details:Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of Frh complex
Details: ::::EMDATABANK.org::::EMD-2096::::
⬡ Geometry
X Y Z
Dimensions 288 288 288
Origin 0 0 0
Spacing 288 288 288
Voxel size 1.14 Å 1.14 Å 1.14 Å
Contour list
Primary Level Source
True 212.0 AUTHOR