EMD-2102
Structure of a stalled transfer intermediate of Sm proteins from the assembly chaperone pICln to the SMN-complex
EMD-2102
Single-particle20.0 Å
Deposition: 17/05/2012
Map released: 30/01/2013
Last modified: 06/03/2013
Concentration: 10
mg/mL
Buffer
pH: 7.5
Details: 20 mM HEPES, 150 mM NaCl, 5 mM DTT
Details: 20 mM HEPES, 150 mM NaCl, 5 mM DTT
Staining
Type:
NEGATIVE
Details: Grids were floated on 2 % Uranyl Formate solution for 1 minute.
Details: Grids were floated on 2 % Uranyl Formate solution for 1 minute.
Grid
Details: Custom made holey grid with thin carbon support.
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 160 kV
Nominal CS: 2 mm
Calibrated magnification: 109000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 160 kV
Nominal CS: 2 mm
Calibrated magnification: 109000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Image Recording
[1]
Detector category:
CCD
Detector model: GENERIC CCD
Number of real images: 48
Average electron dose per image: 20 e/Å2
Bits per pixel: 16.0
Detector model: GENERIC CCD
Number of real images: 48
Average electron dose per image: 20 e/Å2
Bits per pixel: 16.0
Final
reconstruction
Resolution: 20.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 10000
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 10000
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
Imagic | - | - |
CTF correction
Details:Each particle
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the 8S complex
Details: ::::EMDATABANK.org::::EMD-2102::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the 8S complex
Details: ::::EMDATABANK.org::::EMD-2102::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 80 | 80 | 80 |
Origin | 0 | 0 | 0 |
Spacing | 80 | 80 | 80 |
Voxel size | 2.5 Å | 2.5 Å | 2.5 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.017 | AUTHOR |