EMD-2102

Single-particle
20.0 Å
EMD-2102 Deposition: 17/05/2012
Map released: 30/01/2013
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2102

Structure of a stalled transfer intermediate of Sm proteins from the assembly chaperone pICln to the SMN-complex

EMD-2102

Single-particle
20.0 Å
EMD-2102 Deposition: 17/05/2012
Map released: 30/01/2013
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 10 mg/mL
Buffer
pH: 7.5
Details: 20 mM HEPES, 150 mM NaCl, 5 mM DTT
Staining
Type: NEGATIVE
Details: Grids were floated on 2 % Uranyl Formate solution for 1 minute.
Grid
Details: Custom made holey grid with thin carbon support.
Vitrification
Cryogen name: NONE
Instrument: OTHER
Details: Negative stain
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 160 kV
Nominal CS: 2 mm
Calibrated magnification: 109000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Image Recording [1]
Detector category: CCD
Detector model: GENERIC CCD
Number of real images: 48
Average electron dose per image: 20 e/Å2
Bits per pixel: 16.0
Image processing [1]
Final reconstruction
Resolution: 20.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 10000
Algorithm: OTHER
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Imagic - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the 8S complex
Details: ::::EMDATABANK.org::::EMD-2102::::
Geometry
X Y Z
Dimensions 80 80 80
Origin 0 0 0
Spacing 80 80 80
Voxel size 2.5 Å 2.5 Å 2.5 Å
Contour list
Primary Level Source
True 0.017 AUTHOR