EMD-2110

Single-particle
18.0 Å
EMD-2110
3D View Gallery
Deposition: 31/05/2012
Map released: 23/01/2013
Last modified: 23/01/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2110
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Negative structure of open and closed conformation of crm1

EMD-2110

Single-particle
18.0 Å
EMD-2110
3D View Gallery
Deposition: 31/05/2012
Map released: 23/01/2013
Last modified: 23/01/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: 150 mM NaCl, 20 mM HEPES/NaOH pH 7.5, 2 mM MgCl2, 4 mM DTT
Staining
Type: NEGATIVE
Details: Grids with adsorbed protein floated on 1% w/v uranyl formate for 60 seconds
Grid
Details: 200 mesh copper grid with thin carbon support, freshly floated on protein solution
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 160 kV
Nominal magnification: 155000.0
Specimen holder model: OTHER
Temperature
Average: 298 K
Image Recording [1]
Detector category: CCD
Detector model: GENERIC TVIPS (4k x 4k)
Number of real images: 300
Image processing [1]
Final reconstruction
Resolution: 18.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 21000
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Imagic - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Open conformation of Chaetomium thermophilum crm1
Details: ::::EMDATABANK.org::::EMD-2110::::
⬡ Geometry
X Y Z
Dimensions 54 54 54
Origin 0 0 0
Spacing 54 54 54
Voxel size 3.7 Å 3.7 Å 3.7 Å
Contour list
Primary Level Source
True 0.035 EMDB