EMD-2151

Helical reconstruction
22.4 Å
EMD-2151
3D View Gallery
Deposition: 27/06/2012
Map released: 24/10/2012
Last modified: 20/04/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2151
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Electron cryo-microscopy of escrt-III helical polymer

EMD-2151

Helical reconstruction
22.4 Å
EMD-2151
3D View Gallery
Deposition: 27/06/2012
Map released: 24/10/2012
Last modified: 20/04/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Buffer
pH: 7.6
Details: 20 mM Hepes pH 7.6, 150 mM NaCl
Grid
Details: 400 mesh copper quantifoil grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK II
Microscopy [1]
Microscope: FEI TECNAI F30
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.3 mm
Nominal magnification: 39000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 78
Average electron dose per image: 15 e/Å2
Bits per pixel: 8.0
Image processing [1]
Details: The particles were aligned using IHRSR
Final reconstruction
Resolution: 22.4 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details:
⌯ Applied Symmetry
ΔΖ: 6.03 Å
ΔΦ: 11.9°
Axial symmetry: C6
Software [1]
Name Version Details
Ctffind, bsof, spider, imagic - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of chmp2a-3 tubes
Details: ::::EMDATABANK.org::::EMD-2151::::
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin -100 -100 -100
Spacing 200 200 200
Voxel size 3.59 Å 3.59 Å 3.59 Å
Contour list
Primary Level Source
True 1.4 AUTHOR