Electron Microscopy Data Bank

EMD-2159

Single-particle
23.8 Å

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Deposition: 09/07/2012
Map released: 03/10/2012
Last modified: 24/10/2012

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EMD-2159

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Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37

EMD-2159

Single-particle
23.8 Å
EMD-2159

3D View Gallery

Deposition: 09/07/2012
Map released: 03/10/2012
Last modified: 24/10/2012

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

Method: Single-particle
Aggregation State: Particle

Summary:

Specimen preparation
Microscopy
Image processing
Map

Specimen preparation [1]

Buffer

Details: 50 mM Tris pH 7.8, 10 mM MgSO4

Vitrification

Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER

Microscopy [1]

Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 0.5 µm - 5.76 µm
Nominal magnification: 62000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side entry liquid nitrogen-cooled cryo specimen holder
Details: Low dose conditions

Image Recording [1]

Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Number of real images: 297
Average electron dose per image: 15 e/Å²

Image processing [1]

Final reconstruction

Resolution: 23.8 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1028

Applied Symmetry

Point group: I

Software [1]

Name	Version	Details
AUTO3DEM	-	-

CTF correction

Details:Each micrograph

Map

Format: CCP4
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: Three-dimensional reconstruction of the intact (DNA-filled) yersiniophage phiR1-37
Details: ::::EMDATABANK.org::::EMD-2159::::

Geometry

	X	Y	Z
Dimensions	401	401	401
Origin	0	0	0
Spacing	401	401	401
Voxel size	4.516 Å	4.516 Å	4.516 Å

Contour list

Primary	Level	Source
True	12000.0	AUTHOR