EMD-2168
Cryo-EM structure of the 60S-Rei1 complex
EMD-2168
Single-particle14.6 Å
Deposition: 07/08/2012
Map released: 31/10/2012
Last modified: 12/12/2012
Buffer
pH: 8.3
Details: 20 mM Hepes-NaOH pH 8.3, 50 mM NaCl, 5 mM MgCl2, 5 mM beta-mercaptoethanol
Details: 20 mM Hepes-NaOH pH 8.3, 50 mM NaCl, 5 mM MgCl2, 5 mM beta-mercaptoethanol
Staining
Type:
NEGATIVE
Details: Cryo
Details: Cryo
Grid
Details: Quantifoil holey carbon grid R2/1
Vitrification
Cryogen name: ETHANE
Chamber temperature: 80 K
Instrument: HOMEMADE PLUNGER
Method: manual blotting
Details: Vitrification instrument: manual plunger
Chamber temperature: 80 K
Instrument: HOMEMADE PLUNGER
Method: manual blotting
Details: Vitrification instrument: manual plunger
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.3 mm
Nominal defocus: 1.3 µm - 6.0 µm
Nominal magnification: 83000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Minimum tilt angle: 0
Maximum tilt angle: 0
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.3 mm
Nominal defocus: 1.3 µm - 6.0 µm
Nominal magnification: 83000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Minimum tilt angle: 0
Maximum tilt angle: 0
Temperature
Average: 87
K
Image Recording
[1]
Detector category:
CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Sampling interval: 15 µm
Average electron dose per image: 20 e/Å2
Bits per pixel: 16.0
Details: Images were acquired using a 2 x 2 frame spot scan (per hole) using a serial EM script
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Sampling interval: 15 µm
Average electron dose per image: 20 e/Å2
Bits per pixel: 16.0
Details: Images were acquired using a 2 x 2 frame spot scan (per hole) using a serial EM script
Final
reconstruction
Resolution: 14.6
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 17415
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 17415
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
SPIDER | - | - |
CTF correction
Details:Per frame
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of the 60S-Rei1 complex
Details: ::::EMDATABANK.org::::EMD-2168::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of the 60S-Rei1 complex
Details: ::::EMDATABANK.org::::EMD-2168::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 128 | 128 | 128 |
Origin | 0 | 0 | 0 |
Spacing | 128 | 128 | 128 |
Voxel size | 3.17 Å | 3.17 Å | 3.17 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 27.0 | AUTHOR |