EMD-2169

Single-particle
8.1 Å
EMD-2169
3D View Gallery
Deposition: 07/08/2012
Map released: 31/10/2012
Last modified: 10/09/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2169
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 4v8t summary report (pdf.gz) Map and 4v8t FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the 60S-Arx1-Rei1 complex

EMD-2169

Single-particle
8.1 Å
EMD-2169
3D View Gallery
Deposition: 07/08/2012
Map released: 31/10/2012
Last modified: 10/09/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Details: 20 mM Hepes-NaOH pH 8.0, 50 mM NaCl, 5 mM MgCl2, 5 mM beta-mercaptoethanol
Staining
Type: NEGATIVE
Details: Cryo
Grid
Details: Quantifoil holey carbon grid R2/1
Vitrification
Cryogen name: ETHANE
Chamber temperature: 80 K
Instrument: HOMEMADE PLUNGER
Method: manual blotting
Details: Vitrification instrument: manual plunger
Microscopy [3]
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.3 mm
Nominal defocus: 1.5 µm - 4.5 µm
Nominal magnification: 83000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Minimum tilt angle: 0
Maximum tilt angle: 0
Temperature
Average: 87 K
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Sampling interval: 15 µm
Average electron dose per image: 20 e/Å2
Bits per pixel: 16.0
Details: Images were acquired using a 2 x 2 frame spot scan (per hole) using a serial EM script
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.3 mm
Nominal defocus: 1.5 µm - 4.5 µm
Nominal magnification: 83000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Minimum tilt angle: 0
Maximum tilt angle: 0
Temperature
Average: 87 K
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Sampling interval: 15 µm
Average electron dose per image: 20 e/Å2
Bits per pixel: 16.0
Details: Images were acquired using a 2 x 2 frame spot scan (per hole) using a serial EM script
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.3 mm
Nominal defocus: 1.5 µm - 4.5 µm
Nominal magnification: 83000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Minimum tilt angle: 0
Maximum tilt angle: 0
Temperature
Average: 87 K
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Sampling interval: 15 µm
Average electron dose per image: 20 e/Å2
Bits per pixel: 16.0
Details: Images were acquired using a 2 x 2 frame spot scan (per hole) using a serial EM script
Image processing [1]
Final reconstruction
Resolution: 8.1 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 84113
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER - -
CTF correction
Details:Per frame
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of the 60S-Arx1-Rei1 complex
Details: ::::EMDATABANK.org::::EMD-2169::::
⬡ Geometry
X Y Z
Dimensions 224 224 224
Origin 0 0 0
Spacing 224 224 224
Voxel size 1.81 Å 1.81 Å 1.81 Å
Contour list
Primary Level Source
True 35.0 AUTHOR