EMD-2171

Single-particle
24.0 Å
EMD-2171 Deposition: 09/08/2012
Map released: 13/02/2013
Last modified: 13/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2171

Staphylothermus marinus 50S ribosomal subunit

EMD-2171

Single-particle
24.0 Å
EMD-2171 Deposition: 09/08/2012
Map released: 13/02/2013
Last modified: 13/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Staining
Type: NEGATIVE
Details: 20 mM Hepes pH 7.5, 10 mM Mg(OAc)2, 30 mM NH4OAc, 4 mM beta-Mercaptoethanol
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK III
Method: Blot for 10 seconds before plunging, use 2 layers of filter paper
Details: Vitrification instrument: Vitrobot
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 2.0 mm
Nominal magnification: 90000.0
Calibrated magnification: 90000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording [1]
Detector category: FILM
Detector model: FEI EAGLE (4k x 4k)
Scanner: OTHER
Number of real images: 99
Average electron dose per image: 20 e/Å2
Details: Data collected on CCD
Image processing [1]
Final reconstruction
Resolution: 24.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 11142
Algorithm: OTHER
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER - -
CTF correction
Details:Wiener filter on 3D volumes
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Staphylothermus marinus 50S ribosomal subunit
Details: ::::EMDATABANK.org::::EMD-2171::::
Geometry
X Y Z
Dimensions 368 368 368
Origin -184 -184 -183
Spacing 368 368 368
Voxel size 3.31 Å 3.31 Å 3.31 Å
Contour list
Primary Level Source
True 3.61 EMDB