EMD-2172

Single-particle
18.0 Å
EMD-2172
3D View Gallery
Deposition: 09/08/2012
Map released: 13/02/2013
Last modified: 13/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2172
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Methanococcus igneus 70S ribosome

EMD-2172

Single-particle
18.0 Å
EMD-2172
3D View Gallery
Deposition: 09/08/2012
Map released: 13/02/2013
Last modified: 13/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Staining
Type: NEGATIVE
Details: Blot for 10 seconds before plunging, use 2 layers of filter paper
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK III
Method: Blot for 10 seconds before plunging, use 2 layers of filter paper
Details: Vitrification instrument: Vitrobot
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 2.0 mm
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording [1]
Detector category: FILM
Detector model: FEI EAGLE (4k x 4k)
Scanner: OTHER
Number of real images: 99
Average electron dose per image: 20 e/Å2
Details: Data collected on CCD
Image processing [1]
Final reconstruction
Resolution: 18.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 8932
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER - -
CTF correction
Details:Wiener filter on 3D volumes
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Methanococcus igneus 70S ribosome
Details: ::::EMDATABANK.org::::EMD-2172::::
⬡ Geometry
X Y Z
Dimensions 368 368 368
Origin -184 -184 -183
Spacing 368 368 368
Voxel size 3.31 Å 3.31 Å 3.31 Å
Contour list
Primary Level Source
True 2.17 EMDB