EMD-2190

Single-particle
25.0 Å
EMD-2190 Deposition: 10/09/2012
Map released: 16/01/2013
Last modified: 18/09/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2190

human RNA polymerase II in complex with AluRA RNA

EMD-2190

Single-particle
25.0 Å
EMD-2190 Deposition: 10/09/2012
Map released: 16/01/2013
Last modified: 18/09/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.0312 mg/mL
Buffer
pH: 7.9
Details: 10 mM Tris pH 7.9, 10 mM HEPES pH 8.0, 4 mM MgCl2, 50 mM KCl, 0.05% NP-40, 1 mM DTT, 0.1% trehalose
Grid
Details: 400 mesh copper grids covered with a holey carbon film, thin carbon film floated on top
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 86 K
Instrument: FEI VITROBOT MARK II
Method: 6 sec blot, offset -2
Microscopy [1]
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.2 mm
Nominal defocus: 2.0 µm - 5.0 µm
Nominal magnification: 50000.0
Calibrated magnification: 50280.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side-entry cryostage
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Temperature
Average: 78 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 12.7 µm
Number of real images: 228
Average electron dose per image: 20 e/Å2
Old range: 1.0
Bits per pixel: 14.0
Image processing [1]
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 29487
Algorithm: OTHER
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
EMAN2, FREALIGN - -
CTF correction
Details:Whole micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Human RNA polymerase II in complex with AluRA RNA
Details: ::::EMDATABANK.org::::EMD-2190::::
Geometry
X Y Z
Dimensions 146 146 146
Origin 0 0 0
Spacing 146 146 146
Voxel size 2.54 Å 2.54 Å 2.54 Å
Contour list
Primary Level Source
True 0.0666 AUTHOR