EMD-2194
human RNA Polymerase II
EMD-2194
Single-particle25.0 Å
Deposition: 11/09/2012
Map released: 16/01/2013
Last modified: 18/09/2013
Concentration: 0.0312
mg/mL
Buffer
pH: 7.9
Details: 10 mM Tris pH 7.9, 10 mM HEPES pH 8.0, 4 mM MgCl2, 50 mM KCl, 0.05% NP-40, 1 mM DTT, 0.1% trehalose
Details: 10 mM Tris pH 7.9, 10 mM HEPES pH 8.0, 4 mM MgCl2, 50 mM KCl, 0.05% NP-40, 1 mM DTT, 0.1% trehalose
Grid
Details: 400 mesh C-flat grids (Protochips Inc.) with a thin carbon film floated on top
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 86 K
Instrument: FEI VITROBOT MARK II
Method: 6 sec blot, offset -2
Chamber humidity: 100%
Chamber temperature: 86 K
Instrument: FEI VITROBOT MARK II
Method: 6 sec blot, offset -2
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2.2 mm
Nominal defocus: 1.2 µm - 3.0 µm
Nominal magnification: 80000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side-entry cryostage
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2.2 mm
Nominal defocus: 1.2 µm - 3.0 µm
Nominal magnification: 80000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side-entry cryostage
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Temperature
Average: 78
K
Image Recording
[1]
Detector category:
CCD
Detector model: GENERIC GATAN (4k x 4k)
Sampling interval: 1.5 µm
Number of real images: 972
Average electron dose per image: 20 e/Å2
Details: Data collected with leginon
Detector model: GENERIC GATAN (4k x 4k)
Sampling interval: 1.5 µm
Number of real images: 972
Average electron dose per image: 20 e/Å2
Details: Data collected with leginon
Final
reconstruction
Resolution: 25.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 8905
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 8905
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
EMAN2, FREALIGN | - | - |
CTF correction
Details:Whole micrograph
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Human Pol II
Details: ::::EMDATABANK.org::::EMD-2194::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Human Pol II
Details: ::::EMDATABANK.org::::EMD-2194::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 128 | 128 | 128 |
Origin | 0 | 0 | 0 |
Spacing | 128 | 128 | 128 |
Voxel size | 3.01 Å | 3.01 Å | 3.01 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.139 | AUTHOR |