EMD-2194

Single-particle
25.0 Å
EMD-2194 Deposition: 11/09/2012
Map released: 16/01/2013
Last modified: 18/09/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2194

human RNA Polymerase II

EMD-2194

Single-particle
25.0 Å
EMD-2194 Deposition: 11/09/2012
Map released: 16/01/2013
Last modified: 18/09/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.0312 mg/mL
Buffer
pH: 7.9
Details: 10 mM Tris pH 7.9, 10 mM HEPES pH 8.0, 4 mM MgCl2, 50 mM KCl, 0.05% NP-40, 1 mM DTT, 0.1% trehalose
Grid
Details: 400 mesh C-flat grids (Protochips Inc.) with a thin carbon film floated on top
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 86 K
Instrument: FEI VITROBOT MARK II
Method: 6 sec blot, offset -2
Microscopy [1]
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2.2 mm
Nominal defocus: 1.2 µm - 3.0 µm
Nominal magnification: 80000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side-entry cryostage
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Temperature
Average: 78 K
Image Recording [1]
Detector category: CCD
Detector model: GENERIC GATAN (4k x 4k)
Sampling interval: 1.5 µm
Number of real images: 972
Average electron dose per image: 20 e/Å2
Details: Data collected with leginon
Image processing [1]
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 8905
Algorithm: OTHER
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
EMAN2, FREALIGN - -
CTF correction
Details:Whole micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Human Pol II
Details: ::::EMDATABANK.org::::EMD-2194::::
Geometry
X Y Z
Dimensions 128 128 128
Origin 0 0 0
Spacing 128 128 128
Voxel size 3.01 Å 3.01 Å 3.01 Å
Contour list
Primary Level Source
True 0.139 AUTHOR