EMD-2219

Electron Crystallography
8.0 Å
EMD-2219
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Deposition: 12/10/2012
Map released: 14/11/2012
Last modified: 14/11/2012
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EMD-2219
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

Cryo-EM structure of gastric H+,K+-ATPase with bound Rb+

EMD-2219

Electron Crystallography
8.0 Å
EMD-2219
3D View Gallery
Deposition: 12/10/2012
Map released: 14/11/2012
Last modified: 14/11/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Electron Crystallography
Aggregation State: 2D Array
Specimen preparation [1]
Concentration: 8 mg/mL
Details: Crystals grown in dialysis
Buffer
pH: 4.8
Details: 20mM propionate, 1mM ADP, 3mM DTT,pH 4.8-4.9 adjusted by Tris, 1mM MgCl2, 1mM AlCl3, 4mM NaF, 10mM RbCl
Grid
Details: Molybdenum grid with thin carbon support
Vitrification
Cryogen name: NITROGEN
Instrument: LEICA KF80
Details: Vitrification carried out in cold room at 4 degrees Celsius
Crystal formation
Details: Crystals grown in dialysis
Microscopy [1]
Microscope: JEOL KYOTO-3000SFF
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 1.6 mm
Nominal defocus: 0.83 µm - 3.48 µm
Nominal magnification: 40000.0
Calibrated magnification: 39500.0
Specimen holder model: JEOL
Specimen holder details: Helium cooled
Minimum tilt angle: -60
Maximum tilt angle: 60
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 248
Bits per pixel: 12.0
Tilt Series [1]
Axis 1 Axis 2
Min. Max. Inc. Min. Max. Inc. Rotation
-60 ° 60 ° - - - - -
Image processing [1]
Details: Images were processed using MRC suite.
Final reconstruction
Resolution: 8.0 Å ( BY AUTHOR)
Details:
Software [1]
Name Version Details
MRC - -
Crystal parameters
A: 141.0 Å
B: 110.6 Å
C: 320.0 Å
γ: 90.0°
α: 90.0°
β: 90.0°
Plane group: P 2 21 21
CTF correction
Details:Each image
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of H+,K+-ATPase with Rb+
Details: ::::EMDATABANK.org::::EMD-2219::::
⬡ Geometry
X Y Z
Dimensions 61 73 161
Origin -30 -36 -80
Spacing 61 73 161
Voxel size 1.8433 Å 1.9583 Å 2.0000 Å
Contour list
Primary Level Source
True 1.2 AUTHOR