EMD-2226

Single-particle
20.0 Å
EMD-2226
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Deposition: 23/10/2012
Map released: 31/10/2012
Last modified: 26/12/2012
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EMD-2226
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EMICSS annotations (xml)

Single particle analysis of recombinant human anaphase promoting complex (APC/C)

EMD-2226

Single-particle
20.0 Å
EMD-2226
3D View Gallery
Deposition: 23/10/2012
Map released: 31/10/2012
Last modified: 26/12/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Details: 20 mM HEPES NaOH pH 8.0, 200 mM NaCl, 2 mM DTT
Staining
Type: NEGATIVE
Details: Grids stained with 2% (w/v) uranyl acetate
Grid
Details: Quantifoil R1.2/1.3 grids coated with a thin layer of carbon
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 1.2 µm -
Nominal magnification: 50000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F415 (4k x 4k)
Average electron dose per image: 100 e/Å2
Details: On recording each adjacent boxes of 2x2 pixels were averaged
Image processing [1]
Details: Particles were manually selected using the EMAN boxer software. The images were analysed using a combination of IMAGIC and Spider software.
Final reconstruction
Resolution: 20.0 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 5639
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Spider, Imagic - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of recombinant human APC/C
Details: ::::EMDATABANK.org::::EMD-2226::::
⬡ Geometry
X Y Z
Dimensions 140 140 140
Origin -70 -70 -70
Spacing 140 140 140
Voxel size 3.4725 Å 3.4725 Å 3.4725 Å
Contour list
Primary Level Source
True 3.25 AUTHOR