EMD-2226
Single particle analysis of recombinant human anaphase promoting complex (APC/C)
EMD-2226
Single-particle20.0 Å
Deposition: 23/10/2012
Map released: 31/10/2012
Last modified: 26/12/2012
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 1.2 µm -
Nominal magnification: 50000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 1.2 µm -
Nominal magnification: 50000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Image Recording
[1]
Detector category:
CCD
Detector model: TVIPS TEMCAM-F415 (4k x 4k)
Average electron dose per image: 100 e/Å2
Details: On recording each adjacent boxes of 2x2 pixels were averaged
Detector model: TVIPS TEMCAM-F415 (4k x 4k)
Average electron dose per image: 100 e/Å2
Details: On recording each adjacent boxes of 2x2 pixels were averaged
Details: Particles were manually selected using the EMAN boxer software. The images were analysed using a combination of IMAGIC and Spider software.
Final
reconstruction
Resolution: 20.0
Å
(
BY AUTHOR)
Resolution method: OTHER
Number of images used: 5639
Details:
Resolution method: OTHER
Number of images used: 5639
Details:
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
Spider, Imagic | - | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of recombinant human APC/C
Details: ::::EMDATABANK.org::::EMD-2226::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of recombinant human APC/C
Details: ::::EMDATABANK.org::::EMD-2226::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 140 | 140 | 140 |
Origin | -70 | -70 | -70 |
Spacing | 140 | 140 | 140 |
Voxel size | 3.4725 Å | 3.4725 Å | 3.4725 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 3.25 | AUTHOR |