Electron Microscopy Data Bank

EMD-2234

Single-particle
10.5 Å

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Deposition: 20/11/2012
Map released: 16/01/2013
Last modified: 06/03/2013

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EMD-2234

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Electron cryo-microscopy of a head-tailed virus infecting extremely halophilic archaea

EMD-2234

Single-particle
10.5 Å
EMD-2234

3D View Gallery

Deposition: 20/11/2012
Map released: 16/01/2013
Last modified: 06/03/2013

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

Method: Single-particle
Aggregation State: Particle

Summary:

Specimen preparation
Microscopy
Image processing
Map

Specimen preparation [1]

Buffer

pH: 7.2
Details: 20mM Tris-HCl

Grid

Details: Quantifoil holey carbon film on 300 mesh copper grid.

Vitrification

Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER

Microscopy [1]

Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 0.4 µm - 2.9 µm
Nominal magnification: 62000.0
Calibrated magnification: 62000.0
Specimen holder model: GATAN LIQUID NITROGEN

Image Recording [1]

Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Bits per pixel: 12.0

Image processing [1]

Final reconstruction

Resolution: 10.5 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 3530

Applied Symmetry

Point group: I

Software [1]

Name	Version	Details
AUTO3DEM	-	-

Map

Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of archaeal virus HVTV-1
Details: ::::EMDATABANK.org::::EMD-2234::::

Geometry

	X	Y	Z
Dimensions	541	541	541
Origin	0	0	0
Spacing	541	541	541
Voxel size	2.26 Å	2.26 Å	2.26 Å

Contour list

Primary	Level	Source
True	-500.0	EMDB