Electron Microscopy Data Bank

EMD-2240

Subtomogram averaging
28.0 Å

3D View Gallery

Deposition: 07/12/2012
Map released: 26/12/2012
Last modified: 26/12/2012

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2240

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3D structure of myosin filaments isolated from human heart muscles by negative stain electron microscopy and single particle image analysis.

EMD-2240

Subtomogram averaging
28.0 Å
EMD-2240

3D View Gallery

Deposition: 07/12/2012
Map released: 26/12/2012
Last modified: 26/12/2012

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

Method: Subtomogram averaging

Summary:

Specimen preparation
Microscopy
Image processing
Map

Specimen preparation [1]

Vitrification

Cryogen name: NONE
Instrument: OTHER

Microscopy [1]

Microscope: FEI TECNAI 20
Illumination mode: SPOT SCAN
Imaging mode: OTHER
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal magnification: 29000.0
Specimen holder model: OTHER

Image Recording [1]

Detector category: CCD
Detector model: TVIPS TEMCAM-F415 (4k x 4k)
Sampling interval: 17 µm
Number of real images: 237
Average electron dose per image: 100 e/Å²
Bits per pixel: 16.0

Tilt Series [1]

Axis 1			Axis 2
Min.	Max.	Inc.	Min.	Max.	Inc.	Rotation
-	-	-	-	-	-	-

Image processing [1]

Details: Average number of tilts used in the 3D reconstructions: 285.

Final reconstruction

Resolution: 28.0 Å ( BY AUTHOR)
Resolution method: OTHER
Algorithm: OTHER
Details:

Software [1]

Name	Version	Details
Imagic	-	-

Map

Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D map of human cardiac myosin filament
Details: ::::EMDATABANK.org::::EMD-2240::::

Geometry

	X	Y	Z
Dimensions	160	160	160
Origin	-80	-79	-80
Spacing	160	160	160
Voxel size	5.94 Å	5.94 Å	5.94 Å

Contour list

Primary	Level	Source
True	1.5	AUTHOR