EMD-2241

Single-particle
18.1 Å
EMD-2241
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Deposition: 07/12/2012
Map released: 06/03/2013
Last modified: 20/03/2013
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EMD-2241
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Negative stain reconstruction of PG9 Fab in complex with HIV-1 SOSIP gp140 trimer

EMD-2241

Single-particle
18.1 Å
EMD-2241
3D View Gallery
Deposition: 07/12/2012
Map released: 06/03/2013
Last modified: 20/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.03 mg/mL
Buffer
pH: 7.4
Details: 50 mM Tris-HCl, 150 mM NaCl
Staining
Type: NEGATIVE
Details: 30 sec Nano-W
Grid
Details: 400 Cu mesh grid with carbon support, glow discharged at 20 mA for 30 seconds
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal defocus: 0.5 µm - 0.9 µm
Nominal magnification: 100000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Alignment procedure: LEGACY (Astigmatism: Objective astigmatism corrected at 100,000x, Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 55
Details: Images taken from 0 to -55 degrees in 5 degree tilt increments.
Temperature
Minimum: 293 K
Maximum: 293 K
Image Recording [1]
Detector category: CCD
Detector model: GENERIC GATAN (4k x 4k)
Sampling interval: 0.109 µm
Number of real images: 1283
Average electron dose per image: 30 e/Å2
Bits per pixel: 16.0
Details: Images recorded on CCD
Image processing [1]
Details: Particles were picked using DogPicker then processed with Xmipp and EMAN
Final reconstruction
Resolution: 18.1 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 33431
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Xmipp, EMAN - -
CTF correction
Details:Not corrected
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of SOSIP gp140 trimer in complex with PG9 Fab
Details: ::::EMDATABANK.org::::EMD-2241::::
⬡ Geometry
X Y Z
Dimensions 160 160 160
Origin -37 -37 -37
Spacing 160 160 160
Voxel size 2.18 Å 2.18 Å 2.18 Å
Contour list
Primary Level Source
True 1.03 AUTHOR