EMD-2246
Cryo-electron microscopy of phirsl1 jumbo phage
EMD-2246
Single-particle21.0 Å
Deposition: 18/12/2012
Map released: 20/02/2013
Last modified: 20/02/2013
Buffer
pH: 7.5
Details: 50 mM Tris-HCl [pH 7.5], 100 mM NaCl, 10 mM MgSO4
Details: 50 mM Tris-HCl [pH 7.5], 100 mM NaCl, 10 mM MgSO4
Staining
Type:
NEGATIVE
Details: Four microliters of the bacteriophage sample (~0.1 mg/ml) were loaded between the mica-carbon interface. The sample was stained using 2% ammonium molybdate pH 7.5 and air-dried.
Details: Four microliters of the bacteriophage sample (~0.1 mg/ml) were loaded between the mica-carbon interface. The sample was stained using 2% ammonium molybdate pH 7.5 and air-dried.
Vitrification
Microscope: FEI POLARA 300
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.3 mm
Nominal defocus: 1.355 µm - 5.295 µm
Nominal magnification: 31000.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 59000, Electron beam tilt params: )
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.3 mm
Nominal defocus: 1.355 µm - 5.295 µm
Nominal magnification: 31000.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 59000, Electron beam tilt params: )
Image Recording
[1]
Detector category:
FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 52
Average electron dose per image: 20 e/Å2
Bits per pixel: 8.0
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 52
Average electron dose per image: 20 e/Å2
Bits per pixel: 8.0
Final
reconstruction
Resolution: 21.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1228
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1228
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
C6
Software
[1]
Name | Version | Details |
---|---|---|
SPIDER | - | - |
CTF correction
Details:Each particle
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of phirsl1 baseplate
Details: ::::EMDATABANK.org::::EMD-2246::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of phirsl1 baseplate
Details: ::::EMDATABANK.org::::EMD-2246::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 180 | 180 | 180 |
Origin | 0 | 0 | 0 |
Spacing | 180 | 180 | 180 |
Voxel size | 4.52 Å | 4.52 Å | 4.52 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.009 | AUTHOR |