EMD-2251

Single-particle
47.1 Å
EMD-2251 Deposition: 25/12/2012
Map released: 16/01/2013
Last modified: 03/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2251

negative stain single-particle reconstruction of conformation IV of the Ltn1 E3 ubiquitin Ligase

EMD-2251

Single-particle
47.1 Å
EMD-2251 Deposition: 25/12/2012
Map released: 16/01/2013
Last modified: 03/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.01 mg/mL
Buffer
pH: 8.0
Details: 190 mM NaCl, 20 mM Tris, 1mM BME
Staining
Type: NEGATIVE
Details: 3 microliters of sample at a concentration of 0.01 mg/mL was applied to a C-flat grid with 2-micron-diameter holes overlaid by thin 1.5 nm continuous carbon. The specimen was stained with 2% uranyl formate 3 times, then let air-dry.
Grid
Details: C-flat grids overlaid with thin (~1.5 nm) carbon
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2 mm
Nominal defocus: 1.2 µm - 2.6 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Specimen holder details: Single-tilt room temperature
Minimum tilt angle: -55
Maximum tilt angle: 0
Temperature
Average: 298 K
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Number of real images: 489
Average electron dose per image: 15 e/Å2
Image processing [1]
Details: Random-conical tilt reconstruction
Final reconstruction
Resolution: 47.1 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 391
Algorithm: OTHER
Details:
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Spider, Appion - -
Final 2D classification
Number of classes: 1
CTF correction
Details:Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Conformational snapshot IV of Ltn1
Details: ::::EMDATABANK.org::::EMD-2251::::
Geometry
X Y Z
Dimensions 160 160 160
Origin 0 0 0
Spacing 160 160 160
Voxel size 2.18 Å 2.18 Å 2.18 Å
Contour list
Primary Level Source
True 0.8 AUTHOR