EMD-2271

Single-particle
21.0 Å
EMD-2271 Deposition: 03/01/2013
Map released: 13/03/2013
Last modified: 13/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2271

Cryo EM map of the Gaalphaq-PLCbeta3 complex.

EMD-2271

Single-particle
21.0 Å
EMD-2271 Deposition: 03/01/2013
Map released: 13/03/2013
Last modified: 13/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 8.0
Details: 20 mM HEPES pH 8, 200 mM NaCl, 2 mM DTT, 0.9 mM CaCl2, 50 microM GDP, 30 microM AlCl3, 10 mM NaF, 5 mM MgCl2
Grid
Details: Glow-discharged Quantifoil R2/200 mesh grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: Blot for 2 seconds at 3 microliter sample.
Details: Vitrification carried out in liquid nitrogen atmosphere.
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2 mm
Nominal defocus: 1.5 µm - 3.5 µm
Nominal magnification: 50000.0
Calibrated magnification: 71138.0
Specimen holder model: OTHER
Specimen holder details: Used two holders.
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 135,000 times magnification., Electron beam tilt params: )
Temperature
Minimum: 89 K
Average: 89 K
Maximum: 95 K
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Number of real images: 350
Average electron dose per image: 20 e/Å2
Image processing [1]
Final reconstruction
Resolution: 21.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 12673
Algorithm: OTHER
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
EMAN1 - -
Final angle assignment
Details: EMAN1
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the PLCbeta3 distal CTD in contact with the PLCbeta3 catalytic core.
Details: ::::EMDATABANK.org::::EMD-2271::::
Geometry
X Y Z
Dimensions 64 64 64
Origin -32 -32 -32
Spacing 64 64 64
Voxel size 4.48 Å 4.48 Å 4.48 Å
Contour list
Primary Level Source
True 0.844 AUTHOR