EMD-2277

Single-particle
5.1 Å
EMD-2277 Deposition: 08/01/2013
Map released: 23/01/2013
Last modified: 03/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2277

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles

EMD-2277

Single-particle
5.1 Å
EMD-2277 Deposition: 08/01/2013
Map released: 23/01/2013
Last modified: 03/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.2 mg/mL
Buffer
pH: 7.45
Details: 3mM Hepes-KOH, 6.6 mM Tris-acetate pH 7.2, 3 mM NH4Cl, 6.6 mM NH4-acetate, 48 mM K-acetate, 4 mM Mg-acetate, 2.4 mM DTT
Grid
Details: Quantifoil grids (2/2) with 3 nm thin carbon on top
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 90 K
Instrument: FEI VITROBOT MARK II
Method: Blot 2.5 seconds before plunging
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal defocus: 1.178 µm - 3.597 µm
Nominal magnification: 59000.0
Calibrated magnification: 79096.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 59,000 times magnification, Electron beam tilt params: )
Temperature
Minimum: 80 K
Average: 85 K
Maximum: 90 K
Image Recording [1]
Detector category: CCD
Detector model: FEI FALCON I (4k x 4k)
Sampling interval: 14 µm
Number of real images: 360
Average electron dose per image: 16 e/Å2
Details: Every image is the average of sixteen frames recorded by the direct electron detector
Image processing [1]
Details: Use a newly developed statistical movie processing to compensate for beam-induced movement
Final reconstruction
Resolution: 5.1 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 35404
Details: Use a newly developed statistical movie processing approach to compensate for beam-induced movement
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
CTFFIND3, RELION - -
CTF correction
Details:Each image
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of 70S ribosome
Details: ::::EMDATABANK.org::::EMD-2277::::
Geometry
X Y Z
Dimensions 200 200 200
Origin -100 -100 -100
Spacing 200 200 200
Voxel size 1.77 Å 1.77 Å 1.77 Å
Contour list
Primary Level Source
True 0.24 AUTHOR