EMD-2280

Single-particle
30.4 Å
EMD-2280
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Deposition: 16/01/2013
Map released: 23/01/2013
Last modified: 23/01/2013
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EMD-2280
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EMICSS annotations (xml)

Negative stain EM structure of the HOPS tethering complex (conformation 1)

EMD-2280

Single-particle
30.4 Å
EMD-2280
3D View Gallery
Deposition: 16/01/2013
Map released: 23/01/2013
Last modified: 23/01/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: 1 M NaCl, 50 mM Hepes/NaOH, 10% glycerol
Staining
Type: NEGATIVE
Details: Grids with adsorbed Protein floated on 0.07% Uranyl Formate for 1-3h at 4C.
Grid
Details: Glow discharged 400 mesh copper grids with thin carbon support
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: JEOL 1400
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 3.4 mm
Nominal defocus: 0.0013 µm - 0.0017 µm
Nominal magnification: 50000.0
Specimen holder model: JEOL
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 10.5 µm
Number of real images: 920
Average electron dose per image: 4.5 e/Å2
Bits per pixel: 16.0
Image processing [1]
Final reconstruction
Resolution: 30.4 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 2979
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPARX - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of HOPS tethering complex
Details: ::::EMDATABANK.org::::EMD-2280::::
⬡ Geometry
X Y Z
Dimensions 128 128 128
Origin 0 0 0
Spacing 128 128 128
Voxel size 4.2 Å 4.2 Å 4.2 Å
Contour list
Primary Level Source
True 0.018 AUTHOR