EMD-2308

Single-particle
14.0 Å
EMD-2308
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Deposition: 05/02/2013
Map released: 13/03/2013
Last modified: 10/04/2013
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2308
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Structural visualization of key steps in human transcription initiation

EMD-2308

Single-particle
14.0 Å
EMD-2308
3D View Gallery
Deposition: 05/02/2013
Map released: 13/03/2013
Last modified: 10/04/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.06 mg/mL
Buffer
pH: 7.9
Details: 10 mM HEPES, 5% glycerol, 10 mM MgCl2, 50 mM KCl, 1 mM DTT, 0.05% NP-40
Staining
Type: NEGATIVE
Details: Grids with adsorbed protein floated on 2% w/v uranyl formate for 1 minute.
Grid
Details: 200 mesh Cu grid
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2.2 mm
Nominal defocus: 0.5 µm - 1.2 µm
Nominal magnification: 80000.0
Calibrated magnification: 80000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Specimen holder details: Room temp single tilt
Alignment procedure: LEGACY (Astigmatism: objective lens astigmatism was corrected at 250,000 times magnification., Electron beam tilt params: )
Details: Data acquired using Leginon
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Number of real images: 600
Average electron dose per image: 20 e/Å2
Image processing [1]
Final reconstruction
Resolution: 14.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 64712
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
EMAN2, SPARX - -
CTF correction
Details:Whole micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: TBP-TFIIA-TFIIB-DNA-Pol II-TFIIF-TFIIE-TFIIH (close)
Details: ::::EMDATABANK.org::::EMD-2308::::
⬡ Geometry
X Y Z
Dimensions 160 160 160
Origin 0 0 0
Spacing 160 160 160
Voxel size 2.56 Å 2.56 Å 2.56 Å
Contour list
Primary Level Source
True 1.69 AUTHOR