EMD-2347

Electron Crystallography
9.0 Å
EMD-2347
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Deposition: 28/03/2013
Map released: 10/07/2013
Last modified: 06/11/2013
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EMD-2347
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

cryo-EM structure of the NavCt voltage-gated sodium channel

EMD-2347

Electron Crystallography
9.0 Å
EMD-2347
3D View Gallery
Deposition: 28/03/2013
Map released: 10/07/2013
Last modified: 06/11/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Electron Crystallography
Aggregation State: 2D Array
Specimen preparation [1]
Concentration: 4.0 mg/mL
Details: Crystals grown by dialysis
Buffer
pH: 9.0
Details: 50mM glycine-NaOH pH9.0, 200mM NaCl, 4mM MgCl2, 5% glycerol, 5% methyl-2,4-pentanediol, 1.5mM NaN3
Grid
Details: Molybdenum EM grid
Vitrification
Cryogen name: NITROGEN
Instrument: LEICA KF80
Crystal formation
Details: Crystals grown by dialysis
Microscopy [1]
Microscope: JEOL KYOTO-3000SFF
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 1.6 mm
Nominal defocus: 0.91 µm - 3.82 µm
Nominal magnification: 40000.0
Calibrated magnification: 39500.0
Specimen holder model: JEOL
Specimen holder details: Helium cooled, top entry
Minimum tilt angle: 0
Maximum tilt angle: 60
Temperature
Minimum: 4 K
Average: 4 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 77
Average electron dose per image: 20 e/Å2
Bits per pixel: 14.0
Tilt Series [1]
Axis 1 Axis 2
Min. Max. Inc. Min. Max. Inc. Rotation
0 ° 60 ° - - - - -
Image processing [1]
Details: Images were processed using MRC suite
Final reconstruction
Resolution: 9.0 Å ( BY AUTHOR)
Resolution method: OTHER
Details:
Software [1]
Name Version Details
MRC - -
Crystal parameters
A: 115.0 Å
B: 115.0 Å
C: 180.0 Å
γ: 90.0°
α: 90.0°
β: 90.0°
Plane group: P 4
CTF correction
Details:Each micrographs
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM structure of voltage-gated Na+ channel
Details: ::::EMDATABANK.org::::EMD-2347::::
⬡ Geometry
X Y Z
Dimensions 55 55 81
Origin 0 0 -40
Spacing 55 55 81
Voxel size 2.090909 Å 2.090909 Å 2.2222223 Å
Contour list
Primary Level Source
True 1.3 EMDB