EMD-2364

Single-particle
4.4 Å
EMD-2364
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Deposition: 17/04/2013
Map released: 14/08/2013
Last modified: 21/08/2013
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2364
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 4btg summary report (pdf.gz) Map and 4btg FULL report (pdf.gz)
EMICSS annotations (xml)

CryoEM reconstruction of the bacteriophage phi6 procapsid to the near-atomic resolution

EMD-2364

Single-particle
4.4 Å
EMD-2364
3D View Gallery
Deposition: 17/04/2013
Map released: 14/08/2013
Last modified: 21/08/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 10 mg/mL
Buffer
pH: 8.0
Details: 10 mM potassium phosphate, 5 mM MgCl2
Grid
Details: 400 mesh C-flat holey carbon grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 80%
Chamber temperature: 100 K
Instrument: FEI VITROBOT MARK I
Method: Blot for 2 seconds before plunging.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 0.8 µm - 2.2 µm
Nominal magnification: 46000.0
Calibrated magnification: 44739.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 220
Average electron dose per image: 15 e/Å2
Bits per pixel: 16.0
Image processing [1]
Details: The particles were selected using e2boxer (EMAN) and manually pruned in bshow (BSOFT). The initial model was taken from our previous reconstruction at 7A resolution (EMD-2341). The final structure was reconstructed in EMAN.
Final reconstruction
Resolution: 4.4 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 18326
Details:
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
EMAN, BSOFT - -
CTF correction
Details:Particles from each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the wildtype P1247 procapsid of bacteriophage phi6
Details: ::::EMDATABANK.org::::EMD-2364::::
⬡ Geometry
X Y Z
Dimensions 420 420 420
Origin -210 -210 -210
Spacing 420 420 420
Voxel size 1.397 Å 1.397 Å 1.397 Å
Contour list
Primary Level Source
True 2.0 AUTHOR