EMD-2373

Single-particle
14.5 Å
EMD-2373
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Deposition: 14/05/2013
Map released: 31/07/2013
Last modified: 11/09/2013
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EMD-2373
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EMICSS annotations (xml)

ribosome-RelA complex

EMD-2373

Single-particle
14.5 Å
EMD-2373
3D View Gallery
Deposition: 14/05/2013
Map released: 31/07/2013
Last modified: 11/09/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.25 mg/mL
Buffer
pH: 7.45
Details: 5mM Hepes-KOH pH 7.45, 50mM KCl, 10mM NH4Cl, 10mM Mg-acetate, 5mM beta-mercapto-ethanol
Staining
Type: NEGATIVE
Details: Cryo
Grid
Details: Quantifoil grids (2/4) with thin carbon on top
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 90 K
Instrument: FEI VITROBOT MARK I
Method: Blot for 3 seconds before plunging
Microscopy [1]
Microscope: JEOL 2200FS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.0 µm - 4.0 µm
Nominal magnification: 40000.0
Calibrated magnification: 40000.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: corrected at 100k, Electron beam tilt params: )
Specialist optics
Energy filter
Name: In-column Omega filter
Lower energy threshold: 15.0 eV
Upper energy threshold: 20.0 eV
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 7 µm
Number of real images: 425
Average electron dose per image: 10 e/Å2
Bits per pixel: 8.0
Image processing [1]
Details: Particles were selected by combining automated particle picking , multivariate data analysis and visual inspection
Final reconstruction
Resolution: 14.5 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 28791
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
XMIPP, SPIDER - -
CTF correction
Details:Defocus groups
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of ribosome-RelA complex
Details: ::::EMDATABANK.org::::EMD-2373::::
⬡ Geometry
X Y Z
Dimensions 210 210 210
Origin 0 0 0
Spacing 210 210 210
Voxel size 1.75 Å 1.75 Å 1.75 Å
Contour list
Primary Level Source
True 0.175 AUTHOR