EMD-2415

Helical reconstruction
28.0 Å
EMD-2415
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Deposition: 16/07/2013
Map released: 18/12/2013
Last modified: 17/02/2016
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2415
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Helical reconstruction of HMPV matrix protein-lipid filaments

EMD-2415

Helical reconstruction
28.0 Å
EMD-2415
3D View Gallery
Deposition: 16/07/2013
Map released: 18/12/2013
Last modified: 17/02/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Concentration: 0.2 mg/mL
Buffer
pH: 7.5
Details: 20 mM Tris, 650 mM NaCl, 1M NDSB-201
Staining
Type: NEGATIVE
Details: Grids with adsorbed protein-lipid mixtures floated on 2% w/v uranyl acetate for 30 seconds
Grid
Details: 300 mesh copper grid with formvar carbon film
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F30
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 0.72 µm - 0.82 µm
Nominal magnification: 39000.0
Calibrated magnification: 48387.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Temperature
Average: 295 K
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Sampling interval: 15 µm
Number of real images: 1
Bits per pixel: 12.0
Image processing [1]
Details: The reconstruction was calculated using Burnham-Brandeis Helical Package
Final reconstruction
Resolution: 28.0 Å ( BY AUTHOR)
Resolution method: OTHER
Algorithm: OTHER
Details: Final map was filtered to 28 angstrom resolution
⌯ Applied Symmetry
ΔΖ: 5.16 Å
ΔΦ: 56.5°
Software [1]
Name Version Details
Burnham-Brandeis, Helical, Package - -
CTF correction
Details:Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Helical reconstruction of human metapneumovirus matrix protein M bound to DOPC
Details: ::::EMDATABANK.org::::EMD-2415::::
⬡ Geometry
X Y Z
Dimensions 256 256 256
Origin -128 -128 -128
Spacing 256 256 256
Voxel size 3.1 Å 3.1 Å 3.1 Å
Contour list
Primary Level Source
True 2.0 AUTHOR