EMD-2500

Single-particle
25.0 Å
EMD-2500
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Deposition: 23/10/2013
Map released: 25/12/2013
Last modified: 12/10/2016
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2500
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy

EMD-2500

Single-particle
25.0 Å
EMD-2500
3D View Gallery
Deposition: 23/10/2013
Map released: 25/12/2013
Last modified: 12/10/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 8.0
Details: 150mM NaCl, 50mM Tris-HCL,1mM DTT
Staining
Type: NEGATIVE
Details: Negatively stained in 2% (w/v) uranyl acetate solution following the standard deep stain procedure on holey-carbon coated EM copper grids covered with a thin layer of continuous carbon
Grid
Details: 300 mesh continuous carbon
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Specimen holder model: OTHER
Image Recording [1]
Detector category: CCD
Detector model: GENERIC GATAN (4k x 4k)
Number of real images: 300
Average electron dose per image: 30 e/Å2
Image processing [1]
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 24913
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER - -
⦩ Final angle assignment
Details: SPIDER: theta 90 degrees, phi 359.9 degrees
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Exosome incubated with streptavidin labeled ssRNA (50 nucleotides in length)
Details: ::::EMDATABANK.org::::EMD-2500::::
⬡ Geometry
X Y Z
Dimensions 90 90 90
Origin 0 0 0
Spacing 90 90 90
Voxel size 3.0 Å 3.0 Å 3.0 Å
Contour list
Primary Level Source
True 5.0 AUTHOR